MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein.
Fang W, Bartel DP
Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 10.1016/j.molcel.2020.01.026.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164634 | pBI-CMV1-mir144-CMV2-mir1 | Express miR-144 and miR-1-1 in mammalian cells |
| 164635 | pBI-CMV1-mir451-CMV2-mir1 | Express miR-451 and miR-1-1 in mammalian cells |
| 164664 | pBI-CMV1-mir144~451-CMV2-mir1 | Express miR-144~451 and miR-1-1 in mammalian cells |
| 164665 | pBI-CMV1-mir451-CMV2-mir1~144 | Express miR-451 and miR-1-1~144 in mammalian cells |
| 164666 | pBI-CMV1-mir451~144-CMV2-mir1 | Express miR-451~144 and miR-1-1 in mammalian cells |
| 164667 | pBI-CMV1-mir451~451-CMV2-mir1 | Express miR-451~451 and miR-1-1 in mammalian cells |
| 164668 | pBI-CMV1-mir125-CMV2-mir1 | Express miR-125a and miR-1-1 in mammalian cells |
| 164669 | pBI-CMV1-mir125~451-CMV2-mir1 | Express miR-125a~451 and miR-1-1 in mammalian cells |
| 164670 | pBI-CMV1-mir181b-1-CMV2-mir1 | Express miR-181b-1 and miR-1-1 in mammalian cells |
| 164671 | pBI-CMV1-mir181b-1~125-CMV2-mir1 | Express miR-181b-1~125a and miR-1-1 in mammalian cells |
| 164672 | pBI-CMV1-mir374b-CMV2-mir1 | Express miR-374b and miR-1-1 in mammalian cells |
| 164673 | pBI-CMV1-mir421-CMV2-mir1 | Express miR-421 and miR-1-1 in mammalian cells |
| 164674 | pBI-CMV1-mir374b~421-CMV2-mir1 | Express miR-374b~421 and miR-1-1 in mammalian cells |
| 164675 | pBI-CMV1-mir144m1~451-CMV2-mir1 | Express miR-144(mutant1)~451 and miR-1-1 in mammalian cells |
| 164676 | pBI-CMV1-mir144m2~451-CMV2-mir1 | Express miR-144(mutant2)~451 and miR-1-1 in mammalian cells |
| 164677 | pBI-CMV1-mir144m3~451-CMV2-mir1 | Express miR-144(mutant3)~451 and miR-1-1 in mammalian cells |
| 164678 | pBI-CMV1-mir144m4~451-CMV2-mir1 | Express miR-144(mutant4)~451 and miR-1-1 in mammalian cells |
| 164679 | pBI-CMV1-mir144m5~451-CMV2-mir1 | Express miR-144(mutant5)~451 and miR-1-1 in mammalian cells |
| 164680 | pBI-CMV1-mir144[309 nt]~451-CMV2-mir1 | Express miR-144[309 nt]~451 and miR-1-1 in mammalian cells |
| 164681 | pBI-CMV1-mir144[909 nt]~451-CMV2-mir1 | Express miR-144[909 nt]~451 and miR-1-1 in mammalian cells |
| 164682 | pBI-CMV1-mir144[3072 nt]~451-CMV2-mir1 | Express miR-144[3072 nt]~451 and miR-1-1 in mammalian cells |
| 164688 | pLentiCRISPRv1.linker_KO | For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA |
| 164689 | pLentiCRISPRv1.mir451_KO | For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA |
| 164690 | pCK-Drosha(UniProt)-FLAG | Express Drosha-FLAG in mammalian cells |
| 164691 | pCK-Drosha(UniProt)-FLAG-mEGFP | Express Drosha-FLAG-mEGFP in mammalian cells |
| 164692 | pCK-D390(UniProt)-FLAG | Express N-terminally truncated Drosha-FLAG in mammalian cells |
| 164693 | pCK-D390TN(UniProt)-FLAG | Express N-terminally truncated DroshaTN-FLAG in mammalian cells |
| 164694 | pNoTag-DGCR8 | Express DGCR8 without tag in mammalian cells |
| 164791 | pU6-mir144 EF1Alpha-puro-T2A-BFP | Express miR-144 under the U6 promoter in mammalian cells |
| 164792 | pU6-mir451 EF1Alpha-puro-T2A-BFP | Express miR-451 under the U6 promoter in mammalian cells |
| 164793 | pU6-mir144~451 EF1Alpha-puro-T2A-BFP | Express miR-144~451 under the U6 promoter in mammalian cells |
| 164800 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1 | For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1 |
| 164801 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2 | For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2 |