Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans.
Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, Frokjaer-Jensen C
Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159798 | pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR] | Expression construct with ubiquitous expression to test fluorescence level on microscope |
| 159799 | pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR] | Expression construct with ubiquitous expression to test fluorescence level on microscope |
| 159800 | pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR] | Expression construct with ubiquitous expression to test fluorescence level on microscope |
| 159801 | pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR] | Expression construct with ubiquitous expression to test fluorescence level on microscope |
| 159802 | pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR] | Expression construct with ubiquitous expression to test fluorescence level on microscope |
| 159803 | pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR] | Expression construct with ubiquitous expression to test fluorescence level on microscope |
| 159804 | pCFJ1358 - Intron GG1 - smu-1 | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159805 | pCFJ1359 - Intron GG2 - smu-1 | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159806 | pCFJ1360 - Intron GG3 - smu-1 | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159807 | pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159808 | pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159809 | pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159810 | pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159811 | pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159813 | pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159814 | pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159815 | pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159816 | pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR] | Optimized enzyme for genome editing in C. elegans |
| 159817 | pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR] | Positive control for germline GFP expression from simple array in C. elegans |
| 159838 | pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm] | Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays |
| 159843 | pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159844 | pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159845 | pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159846 | pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159847 | pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159848 | pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159849 | pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159850 | pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159851 | pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159852 | pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159853 | pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159854 | pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159855 | pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159856 | pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159857 | pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159858 | pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159859 | pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159860 | pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159861 | pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159862 | pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159863 | pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159864 | pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159865 | pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159866 | pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159867 | pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159869 | pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159870 | pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop) | Three-fragment Gateway compatible flourophore for expression in C. elegans |
| 159872 | pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible protein tag for expression in C. elegans |
| 159873 | pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop) | Three-fragment Gateway compatible protein tag for expression in C. elegans |
| 159874 | pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop) | Three-fragment Gateway compatible protein tag for expression in C. elegans |
| 159877 | pCFJ2369 - intron GG1 - 250bp | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159878 | pCFJ2359 - intron GG2 - 250bp | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159879 | pCFJ2370 - intron GG3 - 250bp | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159880 | pCFJ2214 - Intron GG1 - 900 bp | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159881 | pCFJ2259 - Intron GG2 - 900 bp | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159882 | pCFJ2215 - Intron GG3 - 900 bp | Golden Gate compatible intron with PATCs for reduced germline silencing |
| 159883 | pCFJ2365 - Intron GG1 - 250bp no PATC | Golden Gate compatible intron with no PATCs for negative controls |
| 159884 | pCFJ2345 - Intron GG2 - 250bp no PATC | Golden Gate compatible intron with no PATCs for negative controls |
| 159885 | pCFJ2366 - intron GG3 - 250bp no PATC | Golden Gate compatible intron with no PATCs for negative controls |
| 159886 | pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg) | Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays |
| 159887 | pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start) | Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays |
| 161516 | pCFJ1149 - intron 3 smu-2 PATCs | PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans |