Pooled protein tagging, cellular imaging, and in situ sequencing for monitoring drug action in real time.
Reicher A, Koren A, Kubicek S
Genome Res. 2020 Dec;30(12):1846-1855. doi: 10.1101/gr.261503.120. Epub 2020 Nov 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159740 | Intron-Tagging-EGFP-Donor | Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes. |
| 159741 | Intron-Tagging-pX330-Cas9-Blast | Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid. |
| 159742 | Intron-Tagging-pX330-Cas9-mCherry | Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid. |