Ten catalytic snapshots of rhomboid intramembrane proteolysis from gate opening to peptide release.
Cho S, Baker RP, Ji M, Urban S
Nat Struct Mol Biol. 2019 Oct;26(10):910-918. doi: 10.1038/s41594-019-0296-9. Epub 2019 Sep 30.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155131 | pET15-His-∆NGlpG | Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b |
| 155132 | pET15-His-∆NGlpG(Y205F) | Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b |
| 155133 | pET15-His-∆NGlpG(C104A/M247C) | Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b |
| 155134 | pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G) | Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b |
| 155135 | pGEX-HA-GlpG | Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1 |
| 155136 | pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G) | Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1 |