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An efficient KRAB domain for CRISPRi applications in human cells.

Alerasool N, Segal D, Lee H, Taipale M
Nat Methods. 2020 Nov;17(11):1093-1096. doi: 10.1038/s41592-020-0966-x. Epub 2020 Oct 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154472pLX303-ZIM3-KRAB-dCas9Expresses ZIM3 KRAB domain fused to the N terminus of dCas9
154473pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRABExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3
154474pLX303-ABI1-dCas9Expresses ABI1 fused to the N terminus of dCas9
154761pLX301-ZIM3-KRAB-PYL1Expresses ZIM3 KRAB domain fused to PYL1

Antibodies from Article