The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF.
Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA, Golshani A, Babu M
Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115193 | pLEX307_PDHA2S291D | Lentiviral transduction and expression of PDHA2S291D into any mammalian cell |
| 115194 | pLEX307_PDHA2S293D | Lentiviral transduction and expression of PDHA2S293D into any mammalian cell |
| 115195 | pLEX307_PDHA2S291D/S293D | Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell |
| 115200 | pLEX307_CR-PDHA2S291D | Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3') |
| 115201 | pLEX307_CR-PDHA2S293D | Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3') |
| 115202 | pLEX307_CR-PDHA2S291D/S293D | Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3') |
| 141339 | pLD-puro-Cn-VA-NHEJ1-WT | Lentiviral vector for expression of human NHEJ1-WT in mammalian cells |
| 141340 | pLD-puro-Cn-VA-NHEJ1-S263E | Lentiviral vector for expression of human NHEJ1-S263E in mammalian cells |
| 141341 | pLD-puro-Cn-VA-NHEJ1-S263A | Lentiviral vector for expression of human NHEJ1-S263A in mammalian cells |
| 141342 | pLD-puro-Cn-VA-NHEJ1-S262E/S263E | Lentiviral vector for expression of human NHEJ1-S262E/S263E in mammalian cells |
| 141343 | pLD-puro-Cn-VA-NHEJ1-S262A/S263A | Lentiviral vector for expression of human NHEJ1-S262A/S263A in mammalian cells |