pLD-puro-Cn-VA-NHEJ1-S263E
(Plasmid #141340)

• Purpose: Lentiviral vector for expression of human NHEJ1-S263E in mammalian cells
• Depositing Lab: Mohan Babu
• Publication: Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585.

Backbone

• Vector backbone: pLD-puro-CnVA
• Backbone manufacturer: Addgene # 24587
• Backbone size w/o insert (bp): 9418
• Total vector size (bp): 8598
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Grow for no longer than 16 hr
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NHEJ1-S263E
• Species: H. sapiens (human)
• Insert Size (bp): 1233
• Mutation: S263E
• GenBank ID: 79840
• Entrez Gene: NHEJ1 (a.k.a. XLF)
• Promoter: CMV
• Tag / Fusion Protein:
  • VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BshTI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: 5'-TCTTTCCCCTGCACTGTACC-3'

Resource Information

• Supplemental Documents:
  • pLD-puro-Cn-VA-NHEJ1-S263E.gb
• A portion of this plasmid was derived from a plasmid made by: MGC: 32656, IMAGE: 4076851, GenBank: BC030986.1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry