A quantitative single-cell assay for retrograde membrane traffic enables rapid detection of defects in cellular organization.
Luong P, Li Q, Chen PF, Wrighton PJ, Chang D, Dwyer S, Bayer MT, Snapper SB, Hansen SH, Thiagarajah JT, Goessling W, Lencer WI
Mol Biol Cell. 2019 Nov 27:mbcE19070375. doi: 10.1091/mbc.E19-07-0375.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 141165 | ER-HA-mNG21-10 | Split Fluorescent Reporter for ER Retrograde Trafficking |
| 141166 | CTB | Expresses recombinant Cholera Toxin B Subunit |
| 141167 | CTA2-mNG211 | Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand |
| 141213 | Sec61b-HA-mNG21-10 | Membrane Tethered Split Fluorescent Reporter for ER Retragrade |