CRISPR-based transcriptional activation tool for silent genes in filamentous fungi.

Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM
Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

A subset of these plasmids is included in the Fungal Toolkit for Modular Cloning (FTK) Kit.

Plasmids from Article

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ID	Plasmid	Purpose
138945	pLM-AMA18.0 dCas9-VPR	Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers
138944	pLM-AMA15.0 Cas9	Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers