CRISPR-based transcriptional activation tool for silent genes in filamentous fungi.
Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM
Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3.
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PubMed
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Article
A subset of these plasmids is included in the Fungal Toolkit for Modular Cloning (FTK) Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138944 | pLM-AMA15.0 Cas9 | Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers |
| 138945 | pLM-AMA18.0 dCas9-VPR | Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers |