Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery.

Velazquez E, Lorenzo V, Al-Ramahi Y
ACS Synth Biol. 2019 Sep 20;8(9):2186-2193. doi: 10.1021/acssynbio.9b00293. Epub 2019 Sep 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
138709	pSEVA421-Cas9tr	Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes
138711	pACDura3	Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
138712	pSEVA231-CRISPR	Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array