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BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation.

Edwards DS, Maganti R, Tanksley JP, Luo J, Park JJH, Balkanska-Sinclair E, Ling J, Floyd SR
Cell Rep. 2020 Sep 22;32(12):108166. doi: 10.1016/j.celrep.2020.108166. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
137720pCW57-mCherry-2A-BRD4 Iso ADoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform
137721pCW57-mCherry-2A-BRD4 Iso CDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoform
137722pCW57-mCherry-2A-BRD4 Iso AdelCTDDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)
137723pCW57-mCherry-2A-BRD4 Iso CdelETDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

Antibodies from Article