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Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology.

Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, Procko E
PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136434pCEP4-myc-PDGFRalphaMammalian expression plasmid for c-myc-tagged PDGFRA
136435pCEP4-myc-PDGFRalpha-Y206SMammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)
136436pCEP4-myc-PDGFRalpha-V242KMammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)
136437pcDNA3-sPDGFRalpha-Fc (Legacy)Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
136438pcDNA3-sPDGFRalpha-FcMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
136439pcDNA3-sPDGFRalpha-Fc-Y206SMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)
136440pcDNA3-sPDGFRalpha-Fc-V242KMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)
136445pcDNA3-HCMVgH-8his (TB40/E)Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag
136446pcDNA3-HCMVgH (TB40/E)Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length
136447pcDNA3-HCMVgH-8his (Merlin)Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag
136448pcDNA3-HCMVgH (Merlin)Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length
136449pcDNA3-HCMVgL-FLAG (TB40/E)Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag
136450pcDNA3-HCMVgL-FLAG (Merlin)Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag
136451pcDNA3-HCMVgO-sfGFP (TB40/E)Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP
136452pcDNA3-HCMVgO-sfGFP (Merlin)Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP
136453pcDNA3-HCMVgO-8his (TB40/E)Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag
136454pcDNA3-HCMVgO-8his (Merlin)Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag
136455pCEP4-myc-PDGFRbetaMammalian expression plasmid for c-myc-tagged PDGFRB
136456pCEP4-PDGFRA-reporterMammalian fluorescent reporter plasmid for PDGFRA signaling.

Antibodies from Article