Phosphatidylinositol 4,5-bisphosphate drives Ca(2+)-independent membrane penetration by the tandem C2 domain proteins synaptotagmin-1 and Doc2beta.
Bradberry MM, Bao H, Lou X, Chapman ER
J Biol Chem. 2019 Jul 12;294(28):10942-10953. doi: 10.1074/jbc.RA119.007929. Epub 2019 May 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134690 | pGEX-4T Doc2beta C2AB(125) WT | Encodes cytoplasmic domain of Doc2beta for bacterial expression and purification |
| 134691 | pGEX-4T Doc2beta C2AB(125) H158C | Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling |
| 134692 | pGEX-4T Doc2beta C2AB(125) F222C | Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling |
| 134693 | pGEX-4T Doc2beta C2AB(125) A298C | Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling |
| 134694 | pGEX-4T Doc2beta C2AB(125) G361C | Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling |
| 134695 | pGEX-4T Syt1 C2AB(140) WT | Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification |
| 134696 | pGEX-4T Syt1 C2AB(140) M173C | Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling |
| 134697 | pGEX-4T Syt1 C2AB(140) F234C | Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling |
| 134698 | pGEX-4T Syt1 C2AB(140) V304C | Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling |
| 134699 | pGEX-4T Syt1 C2AB(140) I367C | Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling |