pGEX-4T Doc2beta C2AB(125) A298C
(Plasmid
#134693)
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PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134693 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX4T
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Backbone manufacturerGE Healthcare
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDouble C2 protein beta
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SpeciesR. norvegicus (rat)
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MutationA298C, C145A, C217A, C249A, C290A, C338A, C387A
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Entrez GeneDoc2b
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T Doc2beta C2AB(125) A298C was a gift from Edwin Chapman (Addgene plasmid # 134693 ; http://n2t.net/addgene:134693 ; RRID:Addgene_134693) -
For your References section:
Phosphatidylinositol 4,5-bisphosphate drives Ca(2+)-independent membrane penetration by the tandem C2 domain proteins synaptotagmin-1 and Doc2beta. Bradberry MM, Bao H, Lou X, Chapman ER. J Biol Chem. 2019 Jul 12;294(28):10942-10953. doi: 10.1074/jbc.RA119.007929. Epub 2019 May 30. 10.1074/jbc.RA119.007929 PubMed 31147445
