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Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins.

Foust DJ, Godin AG, Ustione A, Wiseman PW, Piston DW
Biophys J. 2019 Nov 5;117(9):1764-1777. doi: 10.1016/j.bpj.2019.09.028. Epub 2019 Sep 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133854pmApple-EGFP-HRasMammalian expression of HRas N-terminally tagged with mApple and EGFP
133855pEGFP(1-233)-mApple(2-236)-HRas(2-189)Mammalian expression of HRas N-terminally tagged with EGFP and mApple
133856pEGFP-GNB1Mammalian expression of GNB1 N-terminally tagged with EGFP
133857pmApple-GNG2Mammalian expression of GNG2 N-terminally tagged with mApple
133858pCD86-EGFPMammalian expression of CD86 C-terminally tagged with EGFP
133859pCD86-mAppleMammalian expression of CD86 C-terminally tagged with mApple
133860pEGFP-CD86-mAppleMammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

Antibodies from Article