Generation of synthetic nanobodies against delicate proteins.
Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Brauer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA
Nat Protoc. 2020 Apr 8. pii: 10.1038/s41596-020-0304-x. doi: 10.1038/s41596-020-0304-x.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132695 | pRDV5 containing Sb-loop | pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve |
| 132696 | pRDV5 containing Sb-convex | pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve |
| 132697 | pDX_init containing Sb-loop | pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve |
| 132698 | pSb_init containing the TM287/288 nanobody Nb_TM#2 | expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA |
| 132699 | pSb_init containing the MBP sybody Sb_MBP#1 | expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA |
| 132700 | pBXNH3CA_MBP | expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA |
| 132701 | pBXNH3LCA_TM287/288 | expresses TM287/288 in E.coli, to use as positive control in ELISA |