NPM1c impedes CTCF functions through cytoplasmic mislocalization in acute myeloid leukemia.

Wang AJ, Han Y, Jia N, Chen P, Minden MD
Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
131852	pHyg-N1	This is the empty vector control for the mBanana competition experiment
131851	pmBanana-Hyg-CTCF-CtermNES-N1	Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells
131850	pmBanana-Hyg-CTCF-NtermNES-N1	Expresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cells
131849	pHcRed-NPMMLF1nes-C1	Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection
131848	pHcRed-NPM1cNLSdN243-C1	Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection
131847	pHcRed-NPM1cOligodN243-C1	Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection
131838	pHcRed-NPM1cdN243-C1	Expresses the last 50 amino acids of HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection
131835	pFlare9A-CD45i4e5i5mutCBS	A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.
131834	pFlare9A-CD45i4e5i5	A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site.
131825	pLVX-NPM1c-IRES-mCherry	Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction