pFlare9A-CD45i4e5i5mutCBS
(Plasmid #131835)

• Purpose: A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.
• Depositing Lab: Mark Minden
• Publication: Wang et al Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. Epub 2019 Dec 12.

Backbone

• Vector backbone: pFlare9A
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTPRC
• Species: H. sapiens (human)
• Mutation: partial intron 4 full exon 5 and partial intron 5 + mutated CTCF binding site
• Entrez Gene: PTPRC (a.k.a. B220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry