Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa.
Lau CH, Ho JW, Lo PK, Tin C
Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158972 | pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA | Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons |
| 158987 | pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA | Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons |
| 158988 | pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA | Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons |
| 158989 | pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA | Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons |
| 158990 | pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA | Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells |
| 159173 | pAAV-pMecp2-SaCas9-pU6-sgRNA | Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons |
| 159174 | pAAV-pMecp2-dSaCas9-pU6-sgRNA | Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons |