Conserved CxnC motifs in Kaposi's sarcoma-associated herpesvirus ORF66 are required for viral late gene expression and are essential for its interaction with ORF34.
Didychuk AL, Castaneda AF, Kushnir LO, Huang CJ, Glaunsinger BA
J Virol. 2019 Oct 2. pii: JVI.01299-19. doi: 10.1128/JVI.01299-19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130953 | pcDNA4/TO-ORF66-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cells |
| 130954 | pCDNA4/TO-2xStrep-ORF66 1-200 | Expresses KSHV ORF66 a.a. 1-200 with an N-terminal 2xStrep tag in mammalian cells |
| 130955 | pcDNA4/TO-ORF66 200-429-2xStrep | Expresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cells |
| 130957 | pLJM1-zeo-ORF66-2xStrep | Lentiviral vector for expression of KSHV ORF66 with a C-terminal 2xStrep tag |
| 130958 | pLJM1-zeo-ORF30-2xStrep | Lentiviral vector for expression of KSHV ORF30 with a C-terminal 2xStrep tag |
| 130959 | pLJM1-zeo-ORF24-3xFLAG | Lentiviral vector for expression of KSHV ORF24 with a C-terminal 3xFLAG tag |
| 131109 | pcDNA4/TO-ORF66 C427A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131110 | pcDNA4/TO-ORF66 C424A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131111 | pcDNA4/TO-ORF66 C423A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131112 | pcDNA4/TO-ORF66 C399A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131113 | pcDNA4/TO-ORF66 C393A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131114 | pcDNA4/TO-ORF66 C355A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131115 | pcDNA4/TO-ORF66 C344A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131116 | pcDNA4/TO-ORF66 C341A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131117 | pcDNA4/TO-ORF66 C301A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131118 | pcDNA4/TO-ORF66 C298A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131119 | pcDNA4/TO-ORF66 C295A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131120 | pcDNA4/TO-ORF66 C289A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cells |
| 131121 | pcDNA4/TO-ORF66 C244A-2xStrep | Expresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cells |