Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels.
Lolicato M, Riegelhaupt PM, Arrigoni C, Clark KA, Minor DL Jr
Neuron. 2014 Dec 17;84(6):1198-212. doi: 10.1016/j.neuron.2014.11.017. Epub 2014 Dec 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130670 | pPICZ:hTRAAK(W262S) | P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP |
| 130672 | pPICZ:hTRAAK(G124I) | P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP |
| 133077 | pGEMHE:hTRAAK(Q258K) | X. laevis expression vector. It will generate the human TRAAK channel |
| 133078 | pIRES:hTRAAK(W262S) | Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant |
| 133079 | pIRES:hTRAAK(G124I) | Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant |
| 133080 | pIRES:hTRAAK | Mammalian expression vector. It will generate the human TRAAK channel full length |