pPICZ:hTRAAK(W262S)
(Plasmid
#130670)
-
PurposeP. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 130670 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepPICZ
-
Vector typeYeast Expression
Growth in Bacteria
-
Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKCNK4
-
SpeciesH. sapiens (human)
-
MutationN104Q, N108Q, W262S
-
Entrez GeneKCNK4 (a.k.a. FHEIG, K2p4.1, TRAAK, TRAAK1)
-
Tag
/ Fusion Protein
- SNS- 3C_cleavage_site-TAAA-GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer AOX5
- 3′ sequencing primer AOX3 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pPICZ:hTRAAK(W262S) was a gift from Dan Minor (Addgene plasmid # 130670 ; http://n2t.net/addgene:130670 ; RRID:Addgene_130670) -
For your References section:
Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels. Lolicato M, Riegelhaupt PM, Arrigoni C, Clark KA, Minor DL Jr. Neuron. 2014 Dec 17;84(6):1198-212. doi: 10.1016/j.neuron.2014.11.017. Epub 2014 Dec 11. 10.1016/j.neuron.2014.11.017 PubMed 25500157
