A cytosine deaminase for programmable single-base RNA editing.
Abudayyeh OO, Gootenberg JS, Franklin B, Koob J, Kellner MJ, Ladha A, Joung J, Kirchgatterer P, Cox DBT, Zhang F
Science. 2019 Jul 11. pii: science.aax7063. doi: 10.1126/science.aax7063.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130661 | pC0078 RESCUE | pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/ |
| 130662 | pC0079 RESCUE-S | pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S |
| 130663 | pC0096 RESCUE truncation (Cas13 delta 892-1095) | CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE |
| 181954 | pC0090 | Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc |
| 181955 | pC0091 | Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc |