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A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.

Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung R, de March C, Bloom JS, Matsunami H, Kosuri S
Cell Syst. 2019 Mar 27;8(3):254-260.e6. doi: 10.1016/j.cels.2019.02.009. Epub 2019 Mar 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
129456pB-Gaolf-IntegrationpiggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf
129457pB-Ric8b-IntegrationpiggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b
129458pB-RTP1S-IntegrationpiggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S
129459pB-RTP2-IntegrationpiggyBac transposon vector that Inducibly (Tet-On) expresses RTP2
129461pB-MultiplexedReporter-MOR170-1piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

Antibodies from Article