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Biochemical characterization of INTS3 and C9ORF80, two subunits of hNABP1/2 heterotrimeric complex in nucleic acid binding.

Vidhyasagar V, He Y, Guo M, Talwar T, Singh RS, Yadav M, Katselis G, Vizeacoumar FJ, Lukong KE, Wu Y
Biochem J. 2018 Jan 2;475(1):45-60. doi: 10.1042/BCJ20170351. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128415pGEX-6P-1-INTS3-FLExpress INTS3 full-length in E. coli with a GST tag (N-terminal)
128416pGEX-6P-1-INTS3-NExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)
128417pGEX-6P-1-INTS3-CExpress INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)
128418pET28a-C9ORF80Express C9ORF80 in E. coli with a His tag (C-terminal)
128642pET28a-INTS3-CExpress INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

Antibodies from Article