Optimized Assembly of a Multifunctional RNA-Protein Nanostructure in a Cell-Free Gene Expression System.
Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, Simmel FC
Nano Lett. 2018 Apr 11;18(4):2650-2657. doi: 10.1021/acs.nanolett.8b00526. Epub 2018 Mar 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127634 | pSB4k5_RNAnano (r-plasmid) | Encodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP. |
| 127635 | pSB1C3_mTq-Tat (c-plasmid) | Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034. |
| 127636 | pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid) | Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2) |
| 127637 | pSB1C3_YPet-PCP (y-plasmid) | Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034. |