Streamlined Genetic Manipulation of Diverse Bacteroides and Parabacteroides Isolates from the Human Gut Microbiota.
Garcia-Bayona L, Comstock LE
mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 10.1128/mBio.01762-19.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
126617 | pLGB28 | pNBU2-derived shuttle vector for integration at attBT2 sites in Bacteroides chromosomes, inulin selection for use in anbitiotic resistant strains |
126618 | pLGB13 | Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection |
126619 | pLGB29 | Suicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselection |
126620 | pLGB30 | Suicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselection |
126621 | pLGB31 | Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection |