N-terminal fusion of the N-terminal domain of bacterial enzyme I facilitates recombinant expression and purification of the human RNA demethylases FTO and Alkbh5.

Khatiwada B, Purslow JA, Underbakke ES, Venditti V
Protein Expr Purif. 2019 Nov 15;167:105540. doi: 10.1016/j.pep.2019.105540. (Link opens in a new window) PubMed (Link opens in a new window) Article

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ID	Plasmid	Purpose
186466	pET-EIN-FTO(32-505)	Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)
125732	pET-EIN-AlkBH5(66-292)	Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.