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Specific Labeling of Stem Cell Activity in Human Colorectal Organoids Using an ASCL2-Responsive Minigene.

Oost KC, van Voorthuijsen L, Fumagalli A, Lindeboom RGH, Sprangers J, Omerzu M, Rodriguez-Colman MJ, Heinz MC, Verlaan-Klink I, Maurice MM, Burgering BMT, van Rheenen J, Vermeulen M, Snippert HJG
Cell Rep. 2018 Feb 6;22(6):1600-1614. doi: 10.1016/j.celrep.2018.01.033. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
125239pLV 4xSTAR-mTagBFP2-blastFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
136255Tol2 8xSTAR-mNeonGreen. PGK-puroFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Antibodies from Article