Engineered reproductively isolated species drive reversible population replacement.
Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, Perry MW, Akbari OS
Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124999 | OA-986B | Expresses dCas9-VPR driven by the Ubiquitin-63E promoter |
| 125000 | OA-986C | Expresses dCas9-VPR driven by the bottleneck promoter |
| 125001 | OA-986D | Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter |
| 125002 | OA-986E | Expresses dCas9-VP64 driven by the bottleneck promoter |
| 125003 | OA-1045A | Expresses gRNAs targeting hid and eve |
| 125004 | OA-1045B | Expresses gRNAs targeting hid, eve, and hedgehog |
| 125005 | OA-1045C | Expresses gRNAs targeting hid, eve, and wingless |
| 125006 | OA-1045D | Expresses gRNAs targeting hedgehog and wingless |
| 125007 | OA-1045E | Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog |