Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation.
Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr
Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127852 | HM3C NaVSp1_p pET24b | Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein. |
| 127854 | HM3C NaVAe1_p pET24b | Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein. |
| 128075 | NaVSp1 FL pIRES | Mammalian cell expression vector for NaVSp1 bacterial sodium channel. |
| 128076 | NaVAe1 FL pIRES | Mammalian cell expression vector for NaVAe1 bacterial sodium channel. |
| 128077 | NaVSp1 FL DeltaCTD pIRES | Mammalian cell expression vector for NaVSp1 bacterial sodium channel. Residues 1-224. |
| 128078 | NaVAe1 FL DeltaCTD pIRES | Mammalian cell expression vector for NaVAe1 bacterial sodium channel. Residues 1-241. |
| 128079 | NaVSp1 Ae1 CC pIRES | Mammalian cell expression vector for (NaVSp1 1-236 NaVAe1 265-288) chimera bacterial sodium channel. |
| 128080 | NaVSp1 Bh1 CTD pIRES | Mammalian cell expression vector for (NaVSp1 1-220 NaVBh1 236-274) chimera bacterial sodium channel. |
| 128081 | NaVSp1 Ms CTD pIRES | Mammalian cell expression vector for (NaVSp1 1-220 NaVMs 222-274) chimera bacterial sodium channel. |
| 128082 | NaVSp1 Ab1 CTD pIRES | Mammalian cell expression vector for (NaVSp1 1-220 NaVAb1 234-283) chimera bacterial sodium channel. |
| 128083 | NaVSp1 Pz CTD pIRES | Mammalian cell expression vector for (NaVSp1 1-220 NaVPz 224-262) chimera bacterial sodium channel. |
| 128084 | NaVSp1 DeltaCC pIRES | Mammalian cell expression vector for NaVSp1 1-236 chimera bacterial sodium channel. |
| 128085 | NaVAe1 Sp1 CTD pIRES | Mammalian cell expression vector for (NaVAe1 1-243 NaVSp1 220-255) chimera bacterial sodium channel. |
| 128086 | NaVAe1 DeltaCC pIRES | Mammalian cell expression vector for NaVAe1 1-264 chimera bacterial sodium channel. |
| 128087 | HA tag NaVSp1 FL pIRES | Mammalian cell expression vector for NaVSp1 bacterial sodium channel. N-terminal HA tag. |