NaVSp1 Ab1 CTD pIRES
(Plasmid
#128082)
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PurposeMammalian cell expression vector for (NaVSp1 1-220 NaVAb1 234-283) chimera bacterial sodium channel.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepIRES
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNavSp1_Ab1CTD
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SpeciesSilicibacter pomeroyi/Alcanivorax borkumensis
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV For (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NaVSp1 Ab1 CTD pIRES was a gift from Dan Minor (Addgene plasmid # 128082 ; http://n2t.net/addgene:128082 ; RRID:Addgene_128082) -
For your References section:
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL Jr. Cell. 2016 Feb 25;164(5):922-36. doi: 10.1016/j.cell.2016.02.001. 10.1016/j.cell.2016.02.001 PubMed 26919429
