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The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Doyle T, Moncorge O, Bonaventure B, Pollpeter D, Lussignol M, Tauziet M, Apolonia L, Catanese MT, Goujon C, Malim MH
Nat Microbiol. 2018 Dec;3(12):1369-1376. doi: 10.1038/s41564-018-0273-9. Epub 2018 Nov 26. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
139443RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)Lentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoter
139444RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4
139445RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter
139446RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)Lentiviral vector genome to ectopically express Firefly luciferase
139447RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter
139448RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter
139449LentiGuide-NeoLentiviral vector with gRNA scaffold and neomycin selectable marker
139450LentiGuide-Neo-CTRLg1Lentiviral vector with non-targeting gRNA and neomycin selectable marker
139451LentiGuide-Neo-CTRLg2Lentiviral vector with non-targeting gRNA and neomycin selectable marker
139452LentiGuide-Neo-NCOA7g1 (BB34)Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker
139453LentiGuide-Neo-NCOA7g2 (BB35)Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker
139454LentiGuide-Neo-NCOA7g3 (BB36)Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker
139455LentiGuide-Puro-CTRLg1 (CG509)Lentiviral vector with non-targeting gRNA and puromycin selectable marker
139456LentiGuide-Puro-CTRLg2 (CG510)Lentiviral vector with non-targeting gRNA and puromycin selectable marker
139457LentiGuide-Puro-NCOA7g1 (BB22)Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
139458LentiGuide-Puro-NCOA7g2 (BB23)Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
139459LentiGuide-Puro-NCOA7g3 (BB24)Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
139460LentiGuide-Puro-IFITM3g1 (BB15)Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker
139461LentiGuide-Puro-GFPg1 (BB09)Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Antibodies from Article