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An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation.

Lerner AM, Hepperla AJ, Keele GR, Meriesh HA, Yumerefendi H, Restrepo D, Zimmerman S, Bear JE, Kuhlman B, Davis IJ, Strahl BD
Genome Res. 2020 Nov;30(11):1605-1617. doi: 10.1101/gr.264283.120. Epub 2020 Oct 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
122002pNIA-CEN-FLAG-LANS1-Set2FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast
122003pNIA-CEN-mVenus-FLAG-LANS1-Set2mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Antibodies from Article