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Mitofusin 2 regulates neutrophil adhesive migration and the actin cytoskeleton.

Zhou W, Hsu AY, Wang Y, Syahirah R, Wang T, Jeffries J, Wang X, Mohammad H, Seleem MN, Umulis D, Deng Q
J Cell Sci. 2020 Sep 4;133(17). pii: jcs.248880. doi: 10.1242/jcs.248880. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
121993p3E-U6a-U6c-mfn2 guideAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression
121994p3E-U6a-U6c-mfn2 guide#2An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression
121995p3E-U6a-U6c-opa1 guideAn entry vector with U6a and U6c promoter driving opa1 guide RNAs expression
121996pMYs-IRES-mitoRFPExpression of mitoRFP in mammalian cells
121997pMYs-MFN2-IRES-mitoRFPExpression of MFN2 and mitoRFP in mammalial cells
160509plko-shmfn2-mito-GFP-ER tetheringRescue mfn2 knockdown by tethering mitochondria and ER with GFP
160655plko-shmfn2-mfn2-Rescue"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

Antibodies from Article