Mechanical loading of desmosomes depends on the magnitude and orientation of external stress.
Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118714 | Desmoplakin II-YPet(short) | The human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control. |
| 118715 | Desmoplakin II tension sensor (F40-based) | The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry. |
| 118716 | Desmoplakin II no force control (F40-based) | The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent. |
| 118717 | Desmoplakin II donor only control (F40-based tension sensor) | The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET. |
| 118718 | Desmoplakin II donor only control (F40-based no force control) | The donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET. |
| 118719 | Desmoplakin II acceptor only control (F40-based tension sensor) | The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET. |
| 118720 | Desmoplakin II acceptor only control (F40-based no force control) | The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET. |
| 118721 | Desmoplakin II tension sensor (FL-based) | The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry. |
| 118722 | Desmoplakin II donor only control (FL-based no force control) | The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET. |
| 118723 | Desmoplakin II donor only control (FL-based tension sensor) | The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET. |
| 118724 | Desmoplakin II no force control (FL-based) | The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent. |
| 118725 | Desmoplakin I tension sensor (F40-based) | The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry. |
| 118726 | Desmoplakin I no force control (F40-based) | The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent. |
| 119186 | Desmoplakin I tension sensor (F40-based) | The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP. |
| 119187 | Desmoplakin I no force control (F40-based) | The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent. |
| 119188 | Desmoplakin I donor only control (F40-based tension sensor) | The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency. |
| 119189 | Desmoplakin I acceptor only control (F40-based tension sensor) | The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET. |
| 119190 | Desmoplakin I affinity mutant (F40-based tension sensor) | The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association. |