CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming.
Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 10.1016/j.stem.2018.09.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118587 | TetO-FUW-Ezh2 | Expresses Ezh2 from the TRE3G promoter |
| 118588 | TetO-FUW-Foxo1 | Expresses Foxo1 from the TRE3G promoter |
| 118589 | TetO-FUW-Ngn1 | Expresses Ngn1 from the TRE3G promoter |
| 118590 | TetO-FUW-Mecom 1 | Expresses Mecom isoform 1 from the TRE3G promoter |
| 118591 | TetO-FUW-Mecom 2 | Expresses Mecom isoform 2 from the TRE3G promoter |
| 118592 | pHR-EF1Alpha-Tet-on 3G | Expresses Tet-on 3G from the EF1Alpha promoter |
| 118593 | pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1 | Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter |
| 118594 | pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP | Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter |
| 122132 | pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry | Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter |
| 122133 | pHR-PGK-scFv GCN4-sfGFP-VP64 | Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter |