Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter.
Nguyen PT, Lai JY, Lee AT, Kaiser JT, Rees DC
Proc Natl Acad Sci U S A. 2018 Oct 23. pii: 1811003115. doi: 10.1073/pnas.1811003115.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118253 | WT MetNIQ in pBAD | Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay |
| 118254 | WT MetNI in pBAD | Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay |
| 118256 | N295A (EcN) MetNIQ in pBAD | N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay |
| 118257 | N229A (EcQ) MetNIQ in pBAD | N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay |
| 118258 | N295A (EcN), F103A (EcI) MetNIQ in pBAD | N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay |
| 118259 | N295A (EcN), M107A (EcI) MetNIQ in pBAD | N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay |
| 118260 | N295A (EcN), Y160A (EcI) MetNIQ in pBAD | N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay |
| 118261 | N295A (EcN), M163A (EcI) MetNIQ in pBAD | N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay |
| 118268 | N229A Lo-MetQ | N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure |
| 118269 | N295A (EcN), E166Q (EcN) MetNI LmH | N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure |
| 118581 | N295A (EcN), N229A (EcQ) MetNIQ in pBAD | N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay |