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Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors.

Iyer S, Mir A, Vega-Badillo J, Roscoe BP, Ibraheim R, Zhu LJ, Lee J, Liu P, Luk K, Mintzer E, Guo D, Soares de Brito J, Emerson CP Jr, Zamore PD, Sontheimer EJ, Wolfe SA
CRISPR J. 2022 Oct;5(5):685-701. doi: 10.1089/crispr.2022.0058. Epub 2022 Sep 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115909pBS-f1(+)-ACTB-mEGFPdonor to insert EGFP at the ACTB locus in human cells
115910pBS-f1(+)-SEC61B-mEGFPdonor to insert EGFP at the SEC61B locus in human cells
115911pBS-f1(+)-TOMM20-mEGFPdonor to insert EGFP at the TOMM20 locus in human cells
115912pBS-f1(+)-GAPDH-IRES-dTomatodonor to insert IRES-dTomato at the GAPDH locus in human cells
115913pBS-f1(+)-SEC61B-dTomatodonor to insert dTomato at the SEC61B locus in human cells
115914pBS-f1(+)-TOMM20-dTomatodonor to insert dTomato at the TOMM20 locus in human cells
115915pBS-f1(+)-ACTB-iTagRFP-Tdonor to insert iTagRFP-T at the ACTB locus in human cells
115916pBS-f1(-)-ACTB-iTagRFP-Tdonor to insert iTagRFP-T at the ACTB locus in human cells
115917pBS-f1(-)-ACTB-mEGFPdonor to insert EGFP at the ACTB locus in human cells
115918pBS-f1(-)-GAPDH-IRES-EGFPdonor to insert IRES-EGFP at the GAPDH locus in human cells
115919pBS-f1(-)-GAPDH-IRES-dTomatodonor to insert IRES-dTomato at the GAPDH locus in human cells
115920pBS-f1(-)-SEC61B-dTomatodonor to insert dTomato at the SEC61B locus in human cells
115921pBS-f1(-)-SEC61B-mEGFPdonor to insert mEGFP at the SEC61B locus in human cells
115922pBS-f1(-)-TOMM20-dTomatodonor to insert dTomato at the TOMM20 locus in human cells
115923pBS-f1(-)-TOMM20-mEGFPdonor to insert EGFP at the TOMM20 locus in human cells
117111pCVL-UCOE-SFFV-TLR-MCV1Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.
117405pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1SpyCas9 sgRNA 1 targeting TLR2.0
117406pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2SpyCas9 sgRNA 2 targeting TLR2.0
117407pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNANmeCas9 sgRNA targeting TLR2.0
117408pLKO.1-puro-U6-CjeCas9-TLR-MCV1-sgRNACjeCas9 sgRNA targeting TLR2.0
117409pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNASauCas9 sgRNA targeting TLR2.0
117410pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNALbCas12a-gRNA targeting TLR2.0
117411pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNAAsCas12a-gRNA targeting TLR2.0
117412U6-FnCas12a-TLR-MCV1-gRNAFnCas12a-gRNA targeting TLR2.0

Antibodies from Article