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Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.

Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L
Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
113410pCMV-hA3A-BE3Expresses hA3A-BE3 in mammalian cells
113411pCMV-hA3B-BE3Expresses hA3B-BE3 in mammalian cells
113412pCMV-hA3C-BE3Expresses hA3C-BE3 in mammalian cells
113413pCMV-hA3D-BE3Expresses hA3D-BE3 in mammalian cells
113414pCMV-hA3F-BE3Expresses hA3F-BE3 in mammalian cells
113415pCMV-hA3G-BE3Expresses hA3G-BE3 in mammalian cells
113416pCMV-hA3H-BE3Expresses hA3H-BE3 in mammalian cells
113417pCMV-hAID-BE3Expresses hAID-BE3 in mammalian cells
113418pCMV-hA1-BE3Expresses hA1-BE3 in mammalian cells
113419pCMV-mA3-BE3Expresses mA3-BE3 in mammalian cells
113420pCMV-mAID-BE3Expresses mAID-BE3 in mammalian cells
113421pCMV-mA1-BE3Expresses mA1-BE3 in mammalian cells
113422pCMV-cAICDA-BE3Expresses cAICDA-BE3 in mammalian cells
113423pCMV-hA3A-eBE-Y130FExpresses hA3A-eBE-Y130F in mammalian cells
113424pCMV-hA3A-BE3-D131YExpresses hA3A-BE3-D131Y in mammalian cells
113425pCMV-hA3A-BE3-Y132DExpresses hA3A-BE3-Y132D in mammalian cells
113426pCMV-hA3A-BE3-C101SExpresses hA3A-BE3-C101S in mammalian cells
113427pCMV-hA3A-BE3-C106SExpresses hA3A-BE3-C106S in mammalian cells
113428pCMV-hA3A-BE3-Y130FExpresses hA3A-BE3-Y130F in mammalian cells
113429pCMV-hA3A-eBE-Y132DExpresses hA3A-eBE-Y132D in mammalian cells

Antibodies from Article