pCMV-cAICDA-BE3
(Plasmid
#113422)
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PurposeExpresses cAICDA-BE3 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCMV
- Backbone size w/o insert (bp) 3399
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecAICDA-BE3
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Alt namepCMV-cAICDA-XTEN-D10A-SGGS-UGI-SGGS-NLS
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SpeciesIctalurus punctatus (channel catfish)
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Insert Size (bp)5073
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Entrez Geneaicda (a.k.a. aid)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH poly reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-cAICDA-BE3 was a gift from Jia Chen (Addgene plasmid # 113422 ; http://n2t.net/addgene:113422 ; RRID:Addgene_113422) -
For your References section:
Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L. Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198. 10.1038/nbt.4198 PubMed 30125268