Structures of the CRISPR genome integration complex.
Wright AV, Liu JJ, Knott GJ, Doxzen KW, Nogales E, Doudna JA
Science. 2017 Sep 15;357(6356):1113-1118. doi: 10.1126/science.aao0679. Epub 2017 Jul 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113127 | pAW181 | E. coli expression vector for His-MBP-E. coli Cas1 R138A |
| 113175 | pAW152 | E. coli expression vector for untagged E. coli Cas1 K12E + Cas2 |
| 113176 | pAW184 | E. coli expression vector for untagged E. coli Cas1 R112A + Cas2 |
| 113177 | pAW158 | E. coli expression vector for untagged E. coli Cas1 R112E + Cas2 |
| 113178 | pAW188 | E. coli expression vector for untagged E. coli Cas1 R131A + Cas2 |
| 113179 | pAW185 | E. coli expression vector for untagged E. coli Cas1 R132A + Cas2 |
| 113180 | pAW189 | E. coli expression vector for untagged E. coli Cas1 Q136A + Cas2 |
| 113181 | pAW174 | E. coli expression vector for untagged E. coli Cas1 R138A + Cas2 |
| 113182 | pAW150 | E. coli expression vector for untagged E. coli Cas1 S143A + Cas2 |
| 113183 | pAW151 | E. coli expression vector for untagged E. coli Cas1 R146A + Cas2 |
| 113184 | pAW186 | E. coli expression vector for untagged E. coli Cas1 R163A + Cas2 |
| 113185 | pAW156 | E. coli expression vector for untagged E. coli Cas1 K259E + Cas2 |
| 113187 | pAW167 | E. coli expression vector for untagged E. coli Cas1 + Cas2 R40E |
| 113188 | pAW209 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array |
| 113189 | pAW210 | E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array |
| 113233 | pAW211 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA |
| 113234 | pAW212 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC |
| 113235 | pAW213 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant |
| 113236 | pAW214 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant |
| 113237 | pAW215 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant |
| 113238 | pAW216 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant |
| 113239 | pAW217 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant |
| 113240 | pAW218 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip |
| 113241 | pAW227 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant |
| 113242 | pAW228 | E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant |
| 113243 | pAW203 | E. coli expression vector for IHFa E10A + IHFb |
| 113244 | pAW204 | E. coli expression vector for IHFa F13A + IHFb |
| 113245 | pAW205 | E. coli expression vector for IHFa D14A + IHFb |
| 113246 | pAW206 | E. coli expression vector for IHFa K15A + IHFb |
| 113247 | pAW207 | E. coli expression vector for IHFa K20A + IHFb |