Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.

Gu B, Posfai E, Rossant J
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
103882	PCS2+Cas9-mSA	Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos
112848	EasyFusion eGFP	General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor
112849	EasyFusion mCherry	General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor
112850	EasyFusion Halo	General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor
112851	EasyFusion T2A-H2B-GFP	General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor
112852	EasyFusion Halo-mAID	General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein
112853	pR26-CAG-H2B-miRFP703	Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines
113086	EasyFusion T2A-H2B-TagBFP	General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor
113089	EasyFusion T2A-H2B-mCherry	General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor
113096	pX330 spCas9-mSA	pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines
113097	EasyFusion T2A-H2B-miRFP703	General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor
113103	Ctcf-Halo-mAID donor	Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence
113104	Halo-Erk2 donor	Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence
113113	Cdk9-Halo donor	Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence
113115	Gata4-GFP donor	Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence
113116	Id2-Halo donor	Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence
113117	Fgfr2-T2A-H2B-Halo donor	Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence
113118	Dppa1-T2A-H2B-Halo donor	Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence
113119	Gata3-T2A-H2B-eGFP donor	Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence
113120	Pdgfra-T2A-H2B-eGFP donor	Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence
113831	EasyFusion mAID-mCherry with Neo	EasyFusion vector for targeting endogeneous genes with mAID-mCherry
113832	PBS Actb-2A-TIR1-GFP	targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation
122948	PCS2+ Cas9	For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos