RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs.
Trotman JB, Giltmier AJ, Mukherjee C, Schoenberg DR
Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 10.1093/nar/gkx801.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
112705 | pcDNA3-FLAG-RNMT | Expresses N-terminally FLAG-tagged human RNMT in mammalian cells |
112706 | pcDNA3-FLAG-RNMT 1-120 | Expresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cells |
112707 | pcDNA3-FLAG-RNMT 121-476 | Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells |
112708 | pcDNA3-ΔN-RNMT | Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation. |
112709 | pGEX-2T-GST-RNMT | Expresses N-terminally GST-tagged human RNMT in bacterial cells |
112710 | pET28b-His-hCE | Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells |
112818 | pGEX-2T-GST-eIF4E K119A | Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells |