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Extending the Time Domain of Neuronal Silencing with Cryptophyte Anion Channelrhodopsins.

Govorunova EG, Sineshchekov OA, Hemmati R, Janz R, Morelle O, Melkonian M, Wong GK, Spudich JL
eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-0174-18. doi: 10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
111075pFUGW-RapACR-EYFPFast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking
111076pcDNA3.1-PsuACR_353-EYFPAnion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells
111077pcDNA3.1-RaACR_687-EYFPAnion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells
111078pcDNA3.1-RlACR_497-EYFPAnion channelrhodopsin from Rhodomonas lens for expression in mammalian cells
111079pcDNA3.1-R1ACR_653-EYFPAnion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells
111080pcDNA3.1-R1ACR_6367-EYFPAnion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells
119195pFUGW-RapACR_T111C-EYFPFast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking
119196pFUGW-GtACR1_C102A-EYFPStep-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open state

Antibodies from Article