A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis.
Bhabha G, Lee J, Ekiert DC, Gam J, Wilson IA, Dyson HJ, Benkovic SJ, Wright PE
Science. 2011 Apr 8;332(6026):234-8. doi: 10.1126/science.1198542.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110828 | pET27b-ecDHFR-N23PP | Bacterial expression of E.coli DHFR N23PP mutant |
| 110829 | pET27b-ecDHFR-N23PP/S148A | Bacterial expression of E.coli DHFR N23PP/S148A mutant |