Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, Goulet A, Doyon Y
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110624 | pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA | A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA |
| 110625 | MSP1594_2x_NLS | Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) |
| 110626 | U6_sgRNA_CAG_hSt1Cas9_LMD9 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA. |
| 110627 | St1Cas9_LMD-9_sgRNA_pUC19 | U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9). |
| 136651 | U6_sgRNA_CAG_hSt1Cas9_CNRZ1066 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA. |
| 136652 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA | Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA |
| 136653 | U6_sgRNA_CAG_hSt1Cas9_LMG18311 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA. |
| 136654 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA | Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA |
| 136655 | U6_sgRNA_CAG_hSt1Cas9_TH1477 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA. |
| 136656 | U6_sgRNA_CAG_hSt1Cas9_MTH17CL396 | A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA. |
| 136657 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA | Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA |
| 136659 | U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA | Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA |
| 136660 | U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9 | Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA |