Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors.
Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA
Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 10.1038/s41589-018-0168-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108251 | sAJM.1504 | Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B |
| 108253 | sAJM.1505 | Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21 |
| 108254 | sAJM.1506 | Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655 |
| 108512 | pAJM.711 | PPhlF-YFP Reporter. DAPG sensor for use in Marionette strain. |
| 108513 | pAJM.712 | PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain. |
| 108514 | pAJM.713 | PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain. |
| 108515 | pAJM.714 | PVanCC-YFP Reporter. Van sensor for use in Marionette strain. |
| 108516 | pAJM.715 | PTac-YFP Reporter. IPTG sensor for use in Marionette strain. |
| 108517 | pAJM.717 | PTet*-YFP Reporter. aTc sensor for use in Marionette strain. |
| 108518 | pAJM.716 | PBAD-YFP Reporter. Ara sensor for use in Marionette strain. |
| 108519 | pAJM.718 | PBetI-YFP Reporter. Cho sensor for use in Marionette strain. |
| 108520 | pAJM.719 | PTtg-YFP Reporter. Nar sensor for use in Marionette strain. |
| 108521 | pAJM.1459 | P3B5C-YFP Reporter. DHBA sensor for use in Marionette strain. |
| 108522 | pAJM.721 | PSalTTC-YFP Reporter. Sal sensor for use in Marionette strain. |
| 108523 | pAJM.944 | PCin-YFP Reporter. OHC14 sensor for use in Marionette strain. |
| 108524 | pAJM.847 | PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain. |
| 108525 | pAJM.657 | CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain. |
| 108526 | pAJM.474 | LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain. |
| 108527 | pAJM.773 | VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain. |
| 108528 | pAJM.336 | LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain. |
| 108529 | pAJM.011 | TetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain. |
| 108530 | pAJM.677 | AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain. |
| 108531 | pAJM.683 | BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain. |
| 108532 | pAJM.661 | TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain. |
| 108533 | pAJM.690 | PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain. |
| 108534 | pAJM.771 | NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain. |
| 108535 | pAJM.1642 | CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain. |
| 108536 | pAJM.884 | AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain. |
| 108537 | pAJM.969 | MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain. |