Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences.
Boehm V, Gerbracht JV, Marx MC, Gehring NH
Nat Commun. 2016 Dec 5;7:13691. doi: 10.1038/ncomms13691.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108366 | pCI-globin_del5UTR_WT-xrRNA-4H | Expresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108367 | pCI-globin_del5UTR_PTC39-xrRNA-4H | Expresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108368 | pCI-TPI_WT-xrRNA-4H | Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108369 | pCI-TPI_PTC160-xrRNA-4H | Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108370 | pCI-TPI_WT-xrRNA-4MS2-4H | Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites) |
| 108371 | pCI-TPI_WT-4MS2-xrRNA-4H | Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites) |
| 108372 | pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H | Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108373 | pCI-TPI_WT-IL6_3UTR-xrRNA-4H | Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108374 | pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H | Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108375 | pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H | FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108376 | pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H | FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108377 | pcDNA5/FRT/TO-TPI_WT-xrRNA-4H | FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |
| 108378 | pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H | FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites) |