Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences.

Boehm V, Gerbracht JV, Marx MC, Gehring NH
Nat Commun. 2016 Dec 5;7:13691. doi: 10.1038/ncomms13691. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
108378	pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H	FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108377	pcDNA5/FRT/TO-TPI_WT-xrRNA-4H	FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108376	pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H	FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108375	pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H	FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108374	pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H	Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108373	pCI-TPI_WT-IL6_3UTR-xrRNA-4H	Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108372	pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H	Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
108371	pCI-TPI_WT-4MS2-xrRNA-4H	Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)
108370	pCI-TPI_WT-xrRNA-4MS2-4H	Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)
108369	pCI-TPI_PTC160-xrRNA-4H	Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)