In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice.
Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H
Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107307 | CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA | Encoding an SPH activation system |
| 107310 | dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4 | Encoding dCas9-10xGCN4 driven by dSV40 promoter |
| 107311 | EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA | Encoding scFv-p65-HSF1 driven by EF1α promoter |
| 107312 | AAV-hSyn-mCherry-P2A-Cre-WPRE | Encoding hSyn-mCherry-Cre |
| 107313 | AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA | Encoding aCamkII-mCherry-Cre |